Chifukwa chiyani Polyacrylamide imagwiritsidwa ntchito pa electrophoresis ya mapuloteni

Mu sayansi yamakono, protein electrophoresis ndi njira yofunika kwambiri yowunikira ndi kutanthauzira mapuloteni. Pakati pa njira imeneyi paliPolyacrylamide, mankhwala ogwiritsidwa ntchito mosiyanasiyana omwe amagwira ntchito ngati maziko a ma gel matrices omwe amagwiritsidwa ntchito mu ma electrophoresis systems a gel. Kapangidwe kake kapadera ka Polyacrylamide kamapangitsa kuti ikhale chida chofunikira kwambiri kwa ofufuza ndi asayansi omwe akufuna kupeza zovuta za mapuloteni ndi momwe amagwirira ntchito.

Polyacrylamide, yomwe nthawi zambiri imatchedwa PAM, ndi polima yopangidwa ndi acrylamide monomers. Kusinthasintha kwake kwakukulu kumachitika chifukwa cha kuthekera kwake kupanga maunyolo ataliatali, zomwe zimapangitsa kuti pakhale chinthu chofanana ndi gel chomwe chingathe kusunga mamolekyu osiyanasiyana a kukula. Kapangidwe kameneka kamapangitsa polyacrylamide kukhala woyenera kwambiri popanga ma matrices okhala ndi mabowo omwe amagwiritsidwa ntchito mu electrophoresis ya mapuloteni.

Mapuloteni electrophoresis ndi njira yomwe imalekanitsa mapuloteni kutengera mphamvu ndi kukula kwawo. Mwa kuyika chitsanzo cha mapuloteni ku gawo lamagetsi mkati mwa polyacrylamide gel matrix, mapuloteni amasuntha kudzera mu gel pamlingo wosiyana, zomwe zimapangitsa kuti pakhale magulu osiyanasiyana omwe amatha kusanthulidwa ndikuwerengedwa. Kulekanitsidwa kumeneku kumapereka chidziwitso chofunikira pa kuyera kwa mapuloteni, kudziwa kulemera kwa mamolekyulu, komanso kukhalapo kwa isoforms.

Udindo wa Polyacrylamide mu Mapuloteni Electrophoresis

Kusankha polyacrylamide ya protein electrophoresis kumachokera ku momwe imasinthidwira. Asayansi amatha kusintha kuchuluka kwa gel matrix kuti igwirizane ndi mapuloteni amitundu yosiyanasiyana. Kuchuluka kwa gel matrix kumapangitsa kuti ma matrices akhale olimba oyenera kuthetsa mapuloteni ang'onoang'ono, pomwe kuchuluka kochepa kumagwiritsidwa ntchito pa mapuloteni akuluakulu. Kusinthasintha kumeneku kumatsimikizira kuti ofufuza amatha kusintha zoyeserera zawo kuti apeze kulekanitsa ndi kusanthula koyenera.

Polyacrylamide monga mankhwalaFlocculant

Ntchito ya Polyacrylamide siigwira ntchito pa gel electrophoresis. Imapezekanso ngati flocculant m'mafakitale osiyanasiyana, monga kuyeretsa madzi ndi kusamalira madzi otayidwa. Monga flocculant, polyacrylamide imathandiza kusonkhanitsa tinthu tomwe timapachikidwa m'madzi, zomwe zimathandiza kuti tichotsedwe. Khalidweli likuwonetsa mphamvu zosiyanasiyana za mankhwalawa komanso momwe amakhudzira sayansi ndi mafakitale.

Kupita Patsogolo mu Electrophoresis Yochokera ku Polyacrylamide

Zaka zaposachedwapa zawona kupita patsogolo kosalekeza mu njira za electrophoresis zochokera ku polyacrylamide. Native PAGE, SDS-PAGE, ndi two-dimensional gel electrophoresis ndi zitsanzo zochepa chabe za momwe kusinthasintha kwa polyacrylamide kwathandizira kupanga njira zapadera zowunikira kapangidwe ka mapuloteni, kusintha pambuyo pa kumasulira, ndi kuyanjana. Njirazi ndizofunika kwambiri pakufufuza za proteomics ndi kuyesa kupeza mankhwala.

Pankhani yofufuza mapuloteni, polyacrylamide imadziwika ngati mnzake wolimba mtima, zomwe zimathandiza ofufuza kufufuza dziko lovuta la mapuloteni. Udindo wake monga maziko a ma gel matrices mu machitidwe a electrophoresis sungathe kunyalanyazidwa. Kuyambira pakuvumbula njira zochizira matenda mpaka kupanga njira zatsopano zochiritsira, electrophoresis yochokera ku polyacrylamide ikupitilizabe kusintha kupita patsogolo kwa sayansi. Pamene ukadaulo ukupita patsogolo, zodabwitsazi zopanga zitha kusintha, zomwe zimatithandiza kumvetsetsa mapuloteni ndi ntchito zawo zambirimbiri.