Chifukwa Polyacrylamide ntchito mapuloteni electrophoresis

M'malo a sayansi yamakono, protein electrophoresis imayima ngati njira yapangodya yowunikira komanso kuzindikira mapuloteni.Pamtima pa njira imeneyi paliPolyacrylamide, gulu losunthika lomwe limagwira ntchito ngati msana wa matrices a gel omwe amagwiritsidwa ntchito mu machitidwe a gel electrophoresis.Makhalidwe apadera a Polyacrylamide amapangitsa kuti ikhale chida chofunikira kwambiri kwa ofufuza ndi asayansi omwe akufuna kudziwa zovuta zamapuloteni komanso momwe amagwirira ntchito.

Polyacrylamide, yomwe nthawi zambiri imatchedwa PAM, ndi polima wopangidwa kuchokera ku acrylamide monomers.Kusinthasintha kwake kodabwitsa kumatheka chifukwa cha kuthekera kwake kupanga maunyolo aatali, zomwe zimapangitsa kuti pakhale chinthu chonga ngati gel chomwe chimatha kukhala ndi mamolekyu osiyanasiyana.Katunduyu amapangitsa Polyacrylamide kukhala woyenera kupanga ma matrices a porous omwe amagwiritsidwa ntchito mu protein electrophoresis.

Mapuloteni electrophoresis ndi njira yomwe imalekanitsa mapuloteni kutengera mtengo ndi kukula kwawo.Poika chitsanzo cha mapuloteni kumunda wamagetsi mkati mwa polyacrylamide gel matrix, mapuloteni amasuntha kudzera mu gel osakaniza mosiyanasiyana, zomwe zimapangitsa kuti zikhale zosiyana zomwe zingathe kufufuzidwa ndikuwerengedwa.Kupatukanaku kumapereka chidziwitso chofunikira pakuyera kwa mapuloteni, kutsimikiza kulemera kwa maselo, ndi kukhalapo kwa isoforms.

Udindo wa Polyacrylamide mu Protein Electrophoresis

Kusankhidwa kwa polyacrylamide kwa protein electrophoresis kumachokera ku chikhalidwe chake chosinthika.Asayansi amatha kusintha kuchuluka kwa matrix a gel kuti agwirizane ndi mapuloteni amitundu yosiyanasiyana.Kuyika kwakukulu kumapanga matrices olimba oyenerera kuthetsa mapuloteni ang'onoang'ono, pamene zochepetsetsa zimagwiritsidwa ntchito popanga mapuloteni akuluakulu.Kusinthasintha uku kumatsimikizira kuti ochita kafukufuku amatha kusintha zoyeserera zawo kuti akwaniritse kupatukana ndi kusanthula koyenera.

Polyacrylamide ngatiFlocculant

Kugwiritsa ntchito kwa Polyacrylamide kumapitilira gawo lake mu gel electrophoresis.Imapezanso ntchito ngati flocculant m'mafakitale osiyanasiyana, monga kuthira madzi ndi kasamalidwe ka madzi oyipa.Monga flocculant, Polyacrylamide aids mu aggregating inaimitsidwa particles mu zamadzimadzi, kutsogolera awo kuchotsa.Chikhalidwe ichi chikuwonetsa kuthekera kosiyanasiyana kwa gululi komanso kukhudzidwa kwakukulu pa sayansi ndi mafakitale.

Kupititsa patsogolo mu Polyacrylamide-Based Electrophoresis

Zaka zaposachedwa zawona kupita patsogolo kosalekeza kwa njira zama electrophoresis zochokera ku polyacrylamide.Native PAGE, SDS-PAGE, ndi two-dimensional gel electrophoresis ndi zitsanzo zochepa chabe za momwe kusinthika kwa polyacrylamide kwathandizira kupanga njira zapadera zowunikira mapuloteni, kusintha kwa pambuyo pa kumasulira, ndi kuyanjana.Njirazi ndizofunika kwambiri pakufufuza kwa proteinomics komanso kuyesa kupeza mankhwala.

Pankhani yosanthula mapuloteni, polyacrylamide imatuluka ngati mnzake wolimba, zomwe zimapangitsa ofufuza kuti afufuze dziko lovuta kwambiri la mapuloteni.Udindo wake monga maziko a matrices a gel mu machitidwe a electrophoresis sangathe kupitirira.Kuchokera pakuvumbulutsa njira zamatenda mpaka kupanga njira zochiritsira zaposachedwa, electrophoresis yochokera ku polyacrylamide ikupitiliza kukonza kupita patsogolo kwa sayansi.Umisiri waukadaulo ukapita patsogolo, kudabwitsa kopangidwa kumeneku kungasinthe, kukulitsa kumvetsetsa kwathu kwa mapuloteni ndi ntchito zake zambirimbiri.